Application of Polarized Light Microscope

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release time : 2021-04-14 14:31:55

In a polarizing microscope, plane polarized light passes through a birefringent material and then is collected using a second polarizing filter to produce a high-contrast image. This technology has been applied in many fields such as medicine, basic biology, industry, etc., and is used to study rock and minerals.

Medical application

Gout diagnosis

Gout occurs because urate crystals are deposited in the synovial fluid of the joints, causing inflammation and pain. Use a polarized light microscope to examine synovial fluid to diagnose gout. The gout-causing urate crystals have negative and elongated optical characteristics, while the pseudogout-causing pyrophosphate has positive optical characteristics. These differences in crystal orientation and interference color on the polarized light microscope are used to distinguish these crystals.

Amyloid test

Amyloid is produced by the abnormal metabolism of protein, causing it to accumulate in the cell. These proteins can be deposited in various organs, such as the spleen, liver, kidney, brain, etc. These aggregates were not observed in normal cells. Using polarized light, these amyloid structures can be visualized. The presence of amyloid is determined by the appearance of bright green under a polarized light microscope.

Application in basic biology

Therefore, this technology provides a non-invasive method for high-contrast imaging of these tissues and cells. Polarized light microscopes do not require any contrast agents or dyes, can be performed in a non-invasive manner and produce high-contrast images. Polarized light microscopes are used to observe several birefringent or birefringent structures in the body, including teeth, striated bone, muscle tissue, neurons, spindles and actinomycin fibers. By adding dyes, these structures can be contrasted sharply. However, since these are living body structures, this step can cause cell death.

Rock inspection

Polarized light microscope can be used to inspect rock structure and its optical properties. This method can also be used to identify minerals inside rocks. Two methods are used for this.

Transmission Polarized Microscope

In this method, a rock drill is used to cut a piece of rock. Polish one side, then use adhesive to fix the sample to the glass slide. Afterwards, the other side is also polished, and balsam is used to fix it on the protective glass.

Immersion method

In this way, the rock is ground into a fine powder and then sprinkled on a glass slide. The powder is surrounded by the immersion liquid and then added to the cover glass. However, this method destroys the structure of the rock. The advantage is that there is no need to prepare thin rock sections.

Industrial application

Liquid crystal

Liquid crystal is a material with characteristics between liquid and solid. Polarizing microscopes are used to detect special optical patterns and phase defects in liquid crystals. They can also be used to determine whether the crystal is optically positive or negative. Liquid crystal retardation can also be measured using a polarizing microscope.

Polymer Materials

Spherulites are spherical transparent birefringent materials and belong to macromolecular crystals. Polarized light microscope can be used to determine its density and size, and then determine the intensity and transparency.

Food chemicals

Polarization microscopy can be used to determine the optical anisotropy of butter and cream emulsions because they have optical anisotropy. Therefore, using this method, any deviations in emulsion conditions or impurities can be determined.

Glass and ceramics

Polarized light microscope can be used to identify the color, shape, diffraction index and crystal impurities of glass and ceramics to determine their quality and defects.

metal products

It is possible to use a polarized light microscope to inspect the composition, surface impurities and properties of the polished metal sample.

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